A Central Hub of Crispr based Genome Editing
CrisprID | Crispr1419 |
Organism | Bombyx mori |
Gene/Locus (Literature) | BmKMO |
NCBI Gene Name | BmKMO |
Target Sequence | GGCCAGATATGCAGATAGTTCGG |
Target+Mutant_Flanking | ATCATCATAAATTCACCCCTAGGCCAGATATGCAGATAGTTCGGTGGCATCT |
Modification Length | D289 |
Genetic Modification | Deletion |
Location | Exon4 |
Conc. (ng/μl) | 200/500 |
Cell line | Fertilized eggs |
Method/Assay | TA cloning/Sequencing |
Crispr efficiency | 35% |
PRM/crRNA/Vector | T7 |
PRM-cas/vector | Sp6 |
Pubmed | 25013902 |