A Central Hub of Crispr based Genome Editing
CrisprID | Crispr1423 |
Organism | Bombyx mori |
Gene/Locus (Literature) | BmTH |
NCBI Gene Name | Th |
Target Sequence | GGAATCTGATTTCATCCAAGATGG |
Target+Mutant_Flanking | CTAGAGCATCGAATTGGACTCCAGTGACTTGGGAAGGGCGAGTCTCAAGGTG |
Modification Length | D4,+1 |
Genetic Modification | Indels |
Location | Exon5 |
Conc. (ng/μl) | 200/500 |
Cell line | Fertilized eggs |
Method/Assay | TA cloning/Sequencing |
Crispr efficiency | 33.30% |
PRM/crRNA/Vector | T7 |
PRM-cas/vector | Sp6 |
Pubmed | 25013902 |