CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr1424 details


CrisprIDCrispr1424
OrganismBombyx mori
Gene/Locus (Literature)BmTH
NCBI Gene NameTh
Target SequenceGGAATCTGATTTCATCCAAGATGG
Target+Mutant_Flanking

CTAGAGCATCGAATTGGACTCCAGTGACTTGGGAAGGGCGAGTCTCAAGGTG
CTAGAG - - - - - - ggcGGACT - - - tTaACTT - GGAAGGGCGAGTCTCAAGGTG

Modification LengthD15,+5
Genetic ModificationIndels
LocationExon5
Conc. (ng/μl) 200/500
Cell line Fertilized eggs
Method/AssayTA cloning/Sequencing
Crispr efficiency 33.30%
PRM/crRNA/VectorT7
PRM-cas/vectorSp6
Pubmed25013902