CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr1478 details


CrisprIDCrispr1478
OrganismRattus norvegicus
Gene/Locus (Literature)Tmdmt1
NCBI Gene NameSlc11a2
Target SequenceAGTCTTCTGTGTCTGAAGCTGGGACCGT - - - 145bp - - - GAGAGCCGCTGAGGCGTCAGAGTATG
Target+Mutant_Flanking

AAGGAGTCTTCTGTGTCTGAAGCTGGGACCGT - - - 145bp - - -GAGAGCCGCTGAGGCGTCAGAGTATGCCTCT
AAGGAGTCTTCTGTGTCTGAAG - - GGG - CCGT - - - 145bp - - - GAG - - - - - - - - - - - - - - - - - - - TATGCCTCT

Modification LengthD3,D19
Genetic ModificationDeletion
LocationExon11
Conc. (ng/μl) 10/25ng
Cell line Fertilized eggs
Method/AssayT7E1 assay
Crispr efficiency 23%
PRM/crRNA/VectorpUC57
PRM-cas/vectorT7
Pubmed25039742