CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr1486 details


CrisprIDCrispr1486
OrganismRattus norvegicus
Gene/Locus (Literature)Cck
NCBI Gene NameCck
Target SequenceCCAGTCTGGCGCAGCTGTAGCCCCCA - - - 329bp - - - CTCCCCCTCAGGCTTCTGCCGCGGCT
Target+Mutant_Flanking

CTGAGCCAGTCTGGCGCAGCTGTAGCCCCCA - - - 329bp - - -CTCCCCCTCAGGCTTCTGCCGCGGCTCCAAT
CTGAGCCA - - - - - - - - - - - - -- - - - - - - - - - - - - - 329bp - - - CTCCCC - - - - - - CTTCTGCCGCGGCTCCAAT

Modification LengthD26,D6
Genetic ModificationDeletion
LocationExon2
Conc. (ng/μl) 10/25ng
Cell line Fertilized eggs
Method/AssayT7E1 assay
Crispr efficiency 36%
PRM/crRNA/VectorpUC57
PRM-cas/vectorT7
Pubmed25039742