CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr1497 details


CrisprIDCrispr1497
OrganismRattus norvegicus
Gene/Locus (Literature)Nestin
NCBI Gene NameNes
Target SequenceGCCAGACCAGCGACATGGAGGGTTGC - - - 578bp - - - CGTGGCTCACATGGAAAGCTCGCTGG
Target+Mutant_Flanking

CGGGGGCCAGACCAGCGACATGGAGGGTTGC - - - 578bp - - -CGTGGCTCACATGGAAAGCTCGCTGGGTCAG
CGGGGGCCAGACC - - - -- - - - - - AGGGTTGC -- - 578bp - - - CGTGGCTCACATGGAAAGCTCGCTGGGTCAG

Modification LengthD10
Genetic ModificationDeletion
LocationExon1
Conc. (ng/μl) 10/25ng
Cell line Fertilized eggs
Method/AssayT7E1 assay
Crispr efficiency 47%
PRM/crRNA/VectorpUC57
PRM-cas/vectorT7
Pubmed25039742