CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr1506 details


CrisprIDCrispr1506
OrganismRattus norvegicus
Gene/Locus (Literature)Nestin
NCBI Gene NameNes
Target SequenceGCCAGACCAGCGACATGGAGGGTTGC - - - 578bp - - - CGTGGCTCACATGGAAAGCTCGCTGG
Target+Mutant_Flanking

CGGGGGCCAGACCAGCGACATGGAGGGTTGC - - - 578bp - - -CGTGGCTCACATGGAAAGCTCGCTGGGTCAG
CGGGGGCCAGACCAG - - - -- TGGAGGGTTGC- - - 578bp - - - CGTGGCTCACATGGAAA - - - - - - - -- - - - - - -

Modification LengthD5,D79
Genetic ModificationDeletion
LocationExon1
Conc. (ng/μl) 10/25ng
Cell line Fertilized eggs
Method/AssayT7E1 assay
Crispr efficiency 47%
PRM/crRNA/VectorpUC57
PRM-cas/vectorT7
Pubmed25039742