A Central Hub of Crispr based Genome Editing
CrisprID | Crispr1552 |
Organism | Mus musculus |
Gene/Locus (Literature) | Ramp2 |
NCBI Gene Name | Ramp2 |
Target Sequence | GGTGAGCACCGTGTCGGGCCCGG |
Target+Mutant_Flanking | CTGCTGCTGCGGGCGGTGAGCACCGTGTCGGGCCCGGGTGGGAGAT |
Modification Length | NA |
Genetic Modification | NA |
Location | Exon1 |
Conc. (ng/μl) | 50/200 |
Cell line | Fertilized eggs |
Method/Assay | T7E1 assay/Surveyor assay/Sequencing |
Crispr efficiency | 78%/100% |
PRM/crRNA/Vector | pBS-T7- R2gRNA |
PRM-cas/vector | pBS-NFCas9 |
Pubmed | 25042988 |