A Central Hub of Crispr based Genome Editing
| CrisprID | Crispr1553 |
| Organism | Mus musculus |
| Gene/Locus (Literature) | Ramp2 |
| NCBI Gene Name | Ramp2 |
| Target Sequence | GGTGAGCACCGTGTCGGGCCCGG |
| Target+Mutant_Flanking | CTGCTGCTGCGGGCGGTGAGCACCGTGTCGGGCCCGGGTGGGAGAT |
| Modification Length | NA |
| Genetic Modification | NA |
| Location | Exon1 |
| Conc. (ng/μl) | 50/200 |
| Cell line | Fertilized eggs |
| Method/Assay | T7E1 assay/Surveyor assay/Sequencing |
| Crispr efficiency | 78%/100% |
| PRM/crRNA/Vector | pBS-T7- R2gRNA |
| PRM-cas/vector | pBS-NFCas9 |
| Pubmed | 25042988 |