CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr1554 details


CrisprIDCrispr1554
OrganismMus musculus
Gene/Locus (Literature)Ramp2
NCBI Gene NameRamp2
Target SequenceGGTGAGCACCGTGTCGGGCCCGG
Target+Mutant_Flanking

CTGCTGCTGCGGGCGGTGAGCACCGTGTCGGGCCCGGGTGGGAGAT
CTGCTGCTGCGGGCGGTGAGCACCGTGTCGGGCC
CGGGTGGGAGAT

Modification LengthNA
Genetic ModificationNA
LocationExon1
Conc. (ng/μl) 50/200
Cell line Fertilized eggs
Method/AssayT7E1 assay/Surveyor assay/Sequencing
Crispr efficiency 78%/100%
PRM/crRNA/VectorpBS-T7- R2gRNA
PRM-cas/vectorpBS-NFCas9
Pubmed25042988