CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr1565 details


CrisprIDCrispr1565
OrganismMus musculus
Gene/Locus (Literature)Ramp2
NCBI Gene NameRamp2
Target SequenceGGTGAGCACCGTGTCGGGCCCGG
Target+Mutant_Flanking

CTGCTGCTGCGGGCGGTGAGCACCGTGTCGGGCCCGGGTGGGAGAT
CTGCTGCTGCGGGCGGTGAGCACCGTGTC
- - - - CCCGGGTGGGAGAT

Modification LengthNA
Genetic ModificationNA
LocationExon1
Conc. (ng/μl) 50/200
Cell line Fertilized eggs
Method/AssayT7E1 assay/Surveyor assay/Sequencing
Crispr efficiency 78%/100%
PRM/crRNA/VectorpBS-T7- R2gRNA
PRM-cas/vectorpBS-NFCas9
Pubmed25042988