CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr1622 details


CrisprIDCrispr1622
OrganismMus musculus
Gene/Locus (Literature)B2m
NCBI Gene NameB2m
Target SequenceCCACCCGCCTCACATTGAAATCCAAATG . . . 41bp . . . TATGTCCTTCAGCAAGGACTGGTCTTTC
Target+Mutant_Flanking

CAGTTCCACCCGCCTCACATTGAAATCCAAATG . . . 41bp . . .TATGTCCTTCAGCAAGGACTGGTCTTTCTATAT
CAGTTCCACCCGCCTCACATTGAAATCCAAATG . . . 41bp . . . TATGTCCTTCAgGCAAGGACTGGTCTTTCTATAT

Modification Length1
Genetic Modificationinsertion
LocationExon2
Conc. (ng/μl) 5ng/20ng
Cell line Embryo
Method/AssayT7E1 assay/Sequencing
Crispr efficiency 42.80%
PRM/crRNA/VectorPU57
PRM-cas/vectorPST1374
Pubmed24269190