CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr1641 details


CrisprIDCrispr1641
OrganismMus musculus
Gene/Locus (Literature)Il2rg
NCBI Gene NameIl2rg
Target SequenceATCTGATAATAATACATTCCAGGAGTGC . . . 54bp . . . GATATCCAGCTCTACCAGACATTTGTTG
Target+Mutant_Flanking

AAGGTATCTGATAATAATACATTCCAGGAGTGC . . . 54bp . . .GATATCCAGCTCTACCAGACATTTGTTGTCCAG   
AAGGTATCTGATAATAATACATatTCCAGGAGTGC . . . 54bp .. . GATATCCAGCTCTACCAGACATTTGTTGTCCAG

Modification Length2
Genetic Modificationinsertion
LocationExon3
Conc. (ng/μl) 5ng/20ng
Cell line Embryo
Method/AssayT7E1 assay/Sequencing
Crispr efficiency 28.57%
PRM/crRNA/VectorPU57
PRM-cas/vectorPST1374
Pubmed24269190