CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr1648 details


CrisprIDCrispr1648
OrganismMus musculus
Gene/Locus (Literature)Prf1
NCBI Gene NamePrf1
Target SequenceCTCACACTGCCAGCGTAATGTGGCCGCA . . . 35bp . . . GGCAGCTGCTAATATCAATAACGACTGG
Target+Mutant_Flanking

CACAGCTCACACTGCCAGCGTAATGTGGCCGCA . . . 35bp . . .GGCAGCTGCTAATATCAATAACGACTGGCGTGT
CACAGCTCACACTGCCAGCGTAATGTGGCCGCA . . . 35bp . . . GGCAGCTGCTAAT - - - - - - - - - - - - - GGCGTGT

Modification Length D13
Genetic ModificationDeletion
LocationExon2
Conc. (ng/μl) 5ng/20ng
Cell line Embryo
Method/AssayT7E1 assay/Sequencing
Crispr efficiency 14.28%
PRM/crRNA/VectorPU57
PRM-cas/vectorPST1374
Pubmed24269190