CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr1660 details


CrisprIDCrispr1660
OrganismMus musculus
Gene/Locus (Literature)Prf1
NCBI Gene NamePrf1
Target SequenceCTCACACTGCCAGCGTAATGTGGCCGCA . . . 35bp . . . GGCAGCTGCTAATATCAATAACGACTGG
Target+Mutant_Flanking

CACAGCTCACACTGCCAGCGTAATGTGGCCGCA . . . 35bp . . .GGCAGCTGCTAATATCAATAACGACTGGCGTGT
CACAGCTCACACT - - - -- - - tgcca - - - - - GCA . . . 21bp . . . GGCAGCTGCTAATATCAATAACGACTGGCGTGT

Modification Length D17,+5
Genetic Modificationindels
LocationExon2
Conc. (ng/μl) 5ng/20ng
Cell line Embryo
Method/AssayT7E1 assay/Sequencing
Crispr efficiency NA
PRM/crRNA/VectorPU57
PRM-cas/vectorPST1374
Pubmed24269190