CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr1675 details


CrisprIDCrispr1675
OrganismMus musculus
Gene/Locus (Literature)Prkdc
NCBI Gene NamePrkdc
Target SequenceCGAGCTGTTCAGAAACACCAAGGACTGA . . . 22bp . . .GAACTGGAGGAAGTGTGATTCATGGTGG
Target+Mutant_Flanking

GGGATCGAGCTGTTCAGAAACACCAAGGACTGA . . . 22bp . . .GAACTGGAGGAAGTGTGATTCATGGTGGGCCCC
GGGATCGAGCTGTTCAGAAACA - - - AGGACTGA . .. 22bp . . .GAACTGGAGGAAGTGTGATTCATGGTGGGCCCC

Modification LengthD3
Genetic ModificationDeletion
LocationExon37
Conc. (ng/μl) 5ng/20ng
Cell line Embryo
Method/AssayT7E1 assay/Sequencing
Crispr efficiency 71.42%
PRM/crRNA/VectorPU57
PRM-cas/vectorPST1374
Pubmed24269190