CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr1696 details


CrisprIDCrispr1696
OrganismMus musculus
Gene/Locus (Literature)Prkdc
NCBI Gene NamePrkdc
Target SequenceCGAGCTGTTCAGAAACACCAAGGACTGA . . . 22bp . . .GAACTGGAGGAAGTGTGATTCATGGTGG
Target+Mutant_Flanking

GGGATCGAGCTGTTCAGAAACACCAAGGACTGA . . . 22bp . . .GAACTGGAGGAAGTGTGATTCATGGTGGGCCCC
GGGATCGAGCTGTTCAGAAACA - - g - - GACTGA . . . 22bp . ..GAACTGGAGGAAGTGT - - - -- - - - - - GTGGGCCCC

Modification LengthD4,+1
Genetic Modificationindels
LocationExon37
Conc. (ng/μl) 5ng/20ng
Cell line Embryo
Method/AssayT7E1 assay/Sequencing
Crispr efficiency NA
PRM/crRNA/VectorPU57
PRM-cas/vectorPST1374
Pubmed24269190