CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr1709 details


CrisprIDCrispr1709
OrganismMus musculus
Gene/Locus (Literature)Rag1
NCBI Gene NameRag1
Target SequenceGGTGTCTGCTTTGATGGACATGGAAGAA . . . 86bp . . . AATGGGGGATGTGAGTGAGAAGCACGGG
Target+Mutant_Flanking

GCTTTGGTGTCTGCTTTGATGGACATGGAAGAA . . . 86bp . . . AATGGGGGATGTGAGTGAGAAGCACGGGAGTGG
GCTTTGG - - - - aagaaa - - - - GACATGGAAGAA . . . 86bp .. . AATGGGGGATGTGAGTGAGAAGCACGGGAGTGG

Modification LengthD14,+6
Genetic Modificationindels
LocationExon2
Conc. (ng/μl) 5ng/20ng
Cell line Embryo
Method/AssayT7E1 assay/Sequencing
Crispr efficiency 42.80%
PRM/crRNA/VectorPU57
PRM-cas/vectorPST1374
Pubmed24269190