CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr1713 details


CrisprIDCrispr1713
OrganismMus musculus
Gene/Locus (Literature)Rag1
NCBI Gene NameRag1
Target SequenceGGTGTCTGCTTTGATGGACATGGAAGAA . . . 86bp . . . AATGGGGGATGTGAGTGAGAAGCACGGG
Target+Mutant_Flanking

GCTTTGGTGTCTGCTTTGATGGACATGGAAGAA . . . 86bp . . .AATGGGGGATGTGAGTGAGAAGCACGGGAGTGG
GCTTTGGTGTCTGCTTTGATGGA - - -- - - AGAA . . . 86bp . . .AATGGG - - - - - - - - - - - - -- - - - - - - - - - - - - - - - -

Modification LengthD6,D28
Genetic ModificationDeletion
LocationExon2
Conc. (ng/μl) 5ng/20ng
Cell line Embryo
Method/AssayT7E1 assay/Sequencing
Crispr efficiency NA
PRM/crRNA/VectorPU57
PRM-cas/vectorPST1374
Pubmed24269190