CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr1723 details


CrisprIDCrispr1723
OrganismMus musculus
Gene/Locus (Literature)Rag1
NCBI Gene NameRag1
Target SequenceGGTGTCTGCTTTGATGGACATGGAAGAA . . . 86bp . . . AATGGGGGATGTGAGTGAGAAGCACGGG
Target+Mutant_Flanking

GCTTTGGTGTCTGCTTTGATGGACATGGAAGAA . . . 86bp . . .AATGGGGGATGTGAGTGAGAAGCACGGGAGTGG
GCTTTGGTGTCTGCTTTGATGGACATGGAAGAA . . . 86bp . . . AATGGGGGATGTGAGTGAGAA - tggagt - - GGAGTGG

Modification LengthD3,+6
Genetic Modificationindels
LocationExon2
Conc. (ng/μl) 5ng/20ng
Cell line Embryo
Method/AssayT7E1 assay/Sequencing
Crispr efficiency NA
PRM/crRNA/VectorPU57
PRM-cas/vectorPST1374
Pubmed24269190