A Central Hub of Crispr based Genome Editing
CrisprID | Crispr1749 |
Organism | Mus musculus |
Gene/Locus (Literature) | Prf1 |
NCBI Gene Name | Prf1 |
Target Sequence | CTCACACTGCCAGCGTAATGTGGCCGCA . . . 35bp . . . GGCAGCTGCTAATATCAATAACGACTGG |
Target+Mutant_Flanking | CACAGCTCACACTGCCAGCGTAATGTGGCCGCA . . . 35bp . . .GGCAGCTGCTAATATCAATAACGACTGGCGTGT |
Modification Length | D13 |
Genetic Modification | Deletion |
Location | Exon2 |
Conc. (ng/μl) | 5ng/20ng |
Cell line | Embryo |
Method/Assay | T7E1 assay/Sequencing |
Crispr efficiency | NA |
PRM/crRNA/Vector | PU57 |
PRM-cas/vector | PST1374 |
Pubmed | 25326897 |