CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr1774 details


CrisprIDCrispr1774
OrganismMus musculus
Gene/Locus (Literature)Rag1
NCBI Gene NameRag1
Target SequenceGGTGTCTGCTTTGATGGACATGGAAGAA . . . 86bp . . . AATGGGGGATGTGAGTGAGAAGCACGGGAGTGG
Target+Mutant_Flanking

GCTTTGGTGTCTGCTTTGATGGACATGGAAGAA . . . 86bp . . .AATGGGGGATGTGAGTGAGAAGCACGGGAGTGG
GCTTTGGTGTCTGCTTTGATG - ACATGGAAGAA . . . 86bp . .. AATGGGGGATGTGAGTGAGAAGCcACGGGAGTGG

Modification LengthD1,+1
Genetic Modificationindels
LocationExon2
Conc. (ng/μl) 5ng/20ng
Cell line Embryo
Method/AssayT7E1 assay/Sequencing
Crispr efficiency NA
PRM/crRNA/VectorPU57
PRM-cas/vectorPST1374
Pubmed25326897