CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr1781 details


CrisprIDCrispr1781
OrganismMus musculus
Gene/Locus (Literature)B2m
NCBI Gene NameB2m
Target SequenceCCACCCGCCTCACATTGAAATCCAAATG . . . 41bp . . . TATGTCCTTCAGCAAGGACTGGTCTTTC
Target+Mutant_Flanking

CAGTTCCACCCGCCTCACATTGAAATCCAAATG . . . 41bp . . .TATGTCCTTCAGCAAGGACTGGTCTTTCTATAT
CAGTTCCAC
- - - - - - - - - - - - -- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCAAGGACTGGTCTTTCTATAT

Modification LengthD76
Genetic ModificationDeletion
LocationExon2
Conc. (ng/μl) 5ng/20ng
Cell line Embryo
Method/AssayT7E1 assay/Sequencing
Crispr efficiency NA
PRM/crRNA/VectorPU57
PRM-cas/vectorPST1374
Pubmed25326897