CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr1925 details


CrisprIDCrispr1925
OrganismMus musculus
Gene/Locus (Literature)Rosa26
NCBI Gene NameRosa26
Target SequenceCCGCCCTGGGCCTGGGAGAATCC
Target+Mutant_Flanking

GCCTCCTGGCTTCTGAGGACCGCCCTGGGCCTGGGAGAATCCCTTCCCCCTC
GCCTCCTGGCTTCTGAG - - - -- - - - - - GGCCTGGGAGAATCCCTTCCCCCTC

Modification LengthD10
Genetic ModificationDeletion
Locationintron locus
Conc. (ng/μl) 10/100g/ml
Cell line intraperitonial injection
Method/AssayT7E1 Assay
Crispr efficiency 21.9-35%
PRM/crRNA/VectorT3
PRM-cas/vectorhCas9
Pubmed23997119