CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr1931 details


CrisprIDCrispr1931
OrganismMus musculus
Gene/Locus (Literature)Rosa26
NCBI Gene NameRosa26
Target SequenceCCGCCCTGGGCCTGGGAGAATCC
Target+Mutant_Flanking

GCCTCCTGGCTTCTGAGGACCGCCCTGGGCCTGGGAGAATCCCTTCCCCCTC
GCCTCCTGGCT - - -- - - - - - - - - - - - - - - - - TGGGAGAATCCCTTCCCCCTC

Modification LengthD20
Genetic ModificationDeletion
Locationintron locus
Conc. (ng/μl) 10/100g/ml
Cell line intraperitonial injection
Method/AssayT7E1 Assay
Crispr efficiency 21.9-35%
PRM/crRNA/VectorT3
PRM-cas/vectorhCas9
Pubmed23997119