A Central Hub of Crispr based Genome Editing
CrisprID | Crispr1994 |
Organism | Danio rerio |
Gene/Locus (Literature) | gol |
NCBI Gene Name | Slc24a5 |
Target Sequence | GGTCTCTCGCAGGATGTTGCTGG |
Target+Mutant_Flanking | TGTGGGTTTCAGGTCTTGGTCTCTCGCAGGATGTTGCTGGAGCCACGTTTATGGCTGCGGGG |
Modification Length | D22,+6 |
Genetic Modification | indels |
Location | NA |
Conc. (ng/μl) | 50/150pg |
Cell line | Embryo |
Method/Assay | T7E1 Assay |
Crispr efficiency | 80-91% |
PRM/crRNA/Vector | T7 |
PRM-cas/vector | SCP6/T3 |
Pubmed | 23918387 |