CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr1999 details


CrisprIDCrispr1999
OrganismDanio rerio
Gene/Locus (Literature)gol
NCBI Gene NameSlc24a5
Target SequenceGGTCTCTCGCAGGATGTTGCTGG
Target+Mutant_Flanking

TGTGGGTTTCAGGTCTTGGTCTCTCGCAGGATGTTGCTGGAGCCACGTTTATGGCTGCGGGG
 - - - - - - - - - - - -- - - - - - - - - - - - - - - - - - - - - - - TGCTGGAGCCACGTTTATGGCTGCGGGG

Modification LengthD43,+8
Genetic Modificationindels
LocationNA
Conc. (ng/μl) 50/150pg
Cell line Embryo
Method/AssayT7E1 Assay
Crispr efficiency 80-91%
PRM/crRNA/VectorT7
PRM-cas/vectorSCP6/T3
Pubmed23918387