CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr2001 details


CrisprIDCrispr2001
OrganismDanio rerio
Gene/Locus (Literature)ddx19
NCBI Gene Nameddx19
Target SequenceGGCAACAGATTCGTGGGCCCAGG
Target+Mutant_Flanking

TTTAAAAAGAAGGACATGGCAACAGATTCGTGGGCCCAGGCTGTCGATGAACAAGAAGCTGC
TTTAAAAAGAAGGACATGGCAACAGATTCGTGGG
acatggCCCAGGCTGTCGATGAACAAGA

Modification Length6
Genetic Modificationinsertion
LocationNA
Conc. (ng/μl) 150/150pg
Cell line Embryo
Method/AssayT7E1 Assay
Crispr efficiency 87-91%
PRM/crRNA/VectorT7
PRM-cas/vectorSCP6/T3
Pubmed23918387