A Central Hub of Crispr based Genome Editing
| CrisprID | Crispr2006 |
| Organism | Danio rerio |
| Gene/Locus (Literature) | ddx19 |
| NCBI Gene Name | ddx19 |
| Target Sequence | GGCAACAGATTCGTGGGCCCAGG |
| Target+Mutant_Flanking | TTTAAAAAGAAGGACATGGCAACAGATTCGTGGGCCCAGGCTGTCGATGAACAAGAAGCTGC |
| Modification Length | D20,+9 |
| Genetic Modification | indels |
| Location | NA |
| Conc. (ng/μl) | 150/150pg |
| Cell line | Embryo |
| Method/Assay | T7E1 Assay |
| Crispr efficiency | 87-91% |
| PRM/crRNA/Vector | T7 |
| PRM-cas/vector | SCP6/T3 |
| Pubmed | 23918387 |