A Central Hub of Crispr based Genome Editing
CrisprID | Crispr2012 |
Organism | Homo sapiens |
Gene/Locus (Literature) | APC |
NCBI Gene Name | APC |
Target Sequence | CGGGCGGCGCTCGTACTTCTGGCCACTGGGCGAGCGTCTGGCAGGTGAGTGAGGCTGCAGG |
Target+Mutant_Flanking | CAGGAGACGAAGAGCCCGGGCGGCGCTCGTACTTCTGGCCACTGGGCGAGCGTCTGGCAGGTGAGTGAGGCTGCAGGCATTGACGTCTCCTC |
Modification Length | D3,+3 |
Genetic Modification | indels |
Location | NA |
Conc. (ng/μl) | NA |
Cell line | U2OScells |
Method/Assay | T7E1 Assay |
Crispr efficiency | 34.60% |
PRM/crRNA/Vector | pSQT1313 /U6 |
PRM-cas/vector | pSQT1601 |
Pubmed | 24770325 |