CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr2012 details


CrisprIDCrispr2012
OrganismHomo sapiens
Gene/Locus (Literature)APC
NCBI Gene NameAPC
Target SequenceCGGGCGGCGCTCGTACTTCTGGCCACTGGGCGAGCGTCTGGCAGGTGAGTGAGGCTGCAGG
Target+Mutant_Flanking

CAGGAGACGAAGAGCCCGGGCGGCGCTCGTACTTCTGGCCACTGGGCGAGCGTCTGGCAGGTGAGTGAGGCTGCAGGCATTGACGTCTCCTC
CAGGAGACGAAGAGCC
CGGGCGGCGCTCGTACTTCTGGCCACTGGcgaAGCGTCTGGCAGGTGAGTGAGGCTGCAGGCATTGACGTCTCCTC

Modification LengthD3,+3
Genetic Modificationindels
LocationNA
Conc. (ng/μl) NA
Cell line U2OScells
Method/AssayT7E1 Assay
Crispr efficiency 34.60%
PRM/crRNA/VectorpSQT1313 /U6
PRM-cas/vectorpSQT1601
Pubmed24770325