A Central Hub of Crispr based Genome Editing
| CrisprID | Crispr2025 |
| Organism | Homo sapiens |
| Gene/Locus (Literature) | BRCA1 |
| NCBI Gene Name | BRCA1 |
| Target Sequence | CCGAAGCTGACAGATGGGTATTCTTTGAGGGGGGGTAGGGGCGGAACCTGAGAGGCGTAAGG |
| Target+Mutant_Flanking | TGGGAGAGTGGATTTCCGAAGCTGACAGATGGGTATTCTTTGAGGGGGGGTAGGGGCGGAACCTGAGAGGCGTAAGGCGTTGTGAACCCTGG |
| Modification Length | D12 |
| Genetic Modification | Deletion |
| Location | NA |
| Conc. (ng/μl) | NA |
| Cell line | U2OScells |
| Method/Assay | T7E1 Assay |
| Crispr efficiency | 14.10% |
| PRM/crRNA/Vector | pSQT1313 /U6 |
| PRM-cas/vector | pSQT1601 |
| Pubmed | 24770325 |