CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr2049 details


CrisprIDCrispr2049
OrganismHomo sapiens
Gene/Locus (Literature)FANCF
NCBI Gene NameFANCF
Target SequenceCCCATTCGCACGGCTCTGGAGCGGCGGCTGCACAACCAGTGGAGGCAAGAGGGCGGCTTTGG
Target+Mutant_Flanking

TTTGGTCGGCATGGCCCCATTCGCACGGCTCTGGAGCGGCGGCTGCACAACCAGTGGAGGCAAGAGGGCGGCTTTGGGCGGGGTCCAGTTCC
TTTGGTCGGCATGGCCCCATTCGCACGGCTCTGGAGCGGCGGCacactattgcaatgaaaataaatttcctttattagctagaagtgagatctgaaggggcttcatgatgtcccataatttttggcagagggaaaaagatctcagtggtatttgtgagccagggcattggccacaccagccaccaccttttgataggcagcctgcacctgaggagtgcgAGTGGAGGCAAGAGGGCGGCTTTGGGCGGGGTCCAGTTCC

Modification LengthD9,+177
Genetic Modificationindels
LocationNA
Conc. (ng/μl) NA
Cell line U2OScells
Method/AssayT7E1 Assay
Crispr efficiency 27%
PRM/crRNA/VectorpSQT1313 /U6
PRM-cas/vectorpSQT1601
Pubmed24770325