A Central Hub of Crispr based Genome Editing
| CrisprID | Crispr2054 |
| Organism | Homo sapiens |
| Gene/Locus (Literature) | MLH1 |
| NCBI Gene Name | MLH1 |
| Target Sequence | CCGTTGAGCATCTAGACGTTTCCTTGGCTCTTCTGGCGCCAAAATGTCGTTCGTGGCAGGGG |
| Target+Mutant_Flanking | TGGCTGAAGGCACTTCCGTTGAGCATCTAGACGTTTCCTTGGCTCTTCTGGCGCCAAAATGTCGTTCGTGGCAGGGGTTATTCGGCGGCTGG |
| Modification Length | D9 |
| Genetic Modification | Deletion |
| Location | NA |
| Conc. (ng/μl) | NA |
| Cell line | U2OScells |
| Method/Assay | T7E1 Assay |
| Crispr efficiency | 27.90% |
| PRM/crRNA/Vector | pSQT1313 /U6 |
| PRM-cas/vector | pSQT1601 |
| Pubmed | 24770325 |