CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr2081 details


CrisprIDCrispr2081
OrganismHomo sapiens
Gene/Locus (Literature)VEGFA
NCBI Gene NameVEGFA
Target SequenceCCACCTCCTCCCCGGCCGGCGGCGGACAGTGGACGCGGCGGCGAGCCGCGGGCAGGGGCCGG
Target+Mutant_Flanking

CCCCAGCCCCAGCTACCACCTCCTCCCCGGCCGGCGGCGGACAGTGGACGCGGCGGCGAGCCGCGGGCAGGGGCCGGAGCCCGCGCCCGGAG
CCCCAGCCCCAGCTA
CCACCTCCTCCCCGG - - - - - - - - - - - -- - - - -ACGCGGCGGCGAGCCGCGGGCAGGGGCCGGAGCCCGCGCCCGGAG

Modification LengthD17
Genetic ModificationDeletion
LocationNA
Conc. (ng/μl) NA
Cell line U2OScells
Method/AssayT7E1 Assay
Crispr efficiency 5%
PRM/crRNA/VectorpSQT1313 /U6
PRM-cas/vectorpSQT1601
Pubmed24770325