CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr2092 details


CrisprIDCrispr2092
OrganismHomo sapiens
Gene/Locus (Literature)VEGFA
NCBI Gene NameVEGFA
Target SequenceCCACCTCCTCCCCGGCCGGCGGCGGACAGTGGACGCGGCGGCGAGCCGCGGGCAGGGGCCGG
Target+Mutant_Flanking

CCATTCCCTCTTTAGCCAGAGCCGGGGTGTGCAGACGGCAGTCACTAGGGGGCGCTCGGCCACCACAGGGAAGCTGGGTGAATGGAGCGAGC
CCATTCCCTCTTTAG
CCAGAGCCGGGGTGTGCAGACGGCAGTCA - - - - - GGGCGCTCGGCCACCACAGGGAAGCTGGGTGAATGGAGCGAGC

Modification LengthD5
Genetic ModificationDeletion
LocationNA
Conc. (ng/μl) NA
Cell line U2OScells
Method/AssayT7E1 Assay
Crispr efficiency 40%
PRM/crRNA/VectorpSQT1313 /U6
PRM-cas/vectorpSQT1601
Pubmed24770325