CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr2140 details


CrisprIDCrispr2140
OrganismDrosophila melanogaster
Gene/Locus (Literature)w1
NCBI Gene Namew
Target SequenceCCCAAAGATGTCCATATTGTGCC
Target+Mutant_Flanking

CCGGAGCCCGGCTGATTGACCGCCCCAAAGATGTCCATATTGTGCCAGGCATAGGTGAGGTT
CCGGAGCCCGGCTGATTGACCCC
- - - -- AaATGTCCATATTGTGCCAGGCATAGGTGAGGTT

Modification LengthD5,+1
Genetic Modificationindels
LocationExon2
Conc. (ng/μl) 0.5/10_g in 30_l
Cell line Embryo
Method/AssayHRMA
Crispr efficiency 4%
PRM/crRNA/VectorT7
PRM-cas/vectorMLM3613
Pubmed23827738