A Central Hub of Crispr based Genome Editing
CrisprID | Crispr2140 |
Organism | Drosophila melanogaster |
Gene/Locus (Literature) | w1 |
NCBI Gene Name | w |
Target Sequence | CCCAAAGATGTCCATATTGTGCC |
Target+Mutant_Flanking | CCGGAGCCCGGCTGATTGACCGCCCCAAAGATGTCCATATTGTGCCAGGCATAGGTGAGGTT |
Modification Length | D5,+1 |
Genetic Modification | indels |
Location | Exon2 |
Conc. (ng/μl) | 0.5/10_g in 30_l |
Cell line | Embryo |
Method/Assay | HRMA |
Crispr efficiency | 4% |
PRM/crRNA/Vector | T7 |
PRM-cas/vector | MLM3613 |
Pubmed | 23827738 |