CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr2179 details


CrisprIDCrispr2179
OrganismDanio rerio
Gene/Locus (Literature)C13H9orf72(C9t3)
NCBI Gene NameC13H9orf72(C9t3)
Target SequenceCCTATCATGTCTTCAGCCTGTCC
Target+Mutant_Flanking

TATAAGCCCCTATCATGTCTTCAGCCTGTCCTCCACAATCTCCAGCCGTGGCC
TATAAGCCCCTAT - - - - - - - ATAAT - - - - - - - - - - CAATCTCCAGCCGTGGCC

Modification LengthNA
Genetic Modificationindels
LocationIntron1
Conc. (ng/μl) 2.4/0.5
Cell line Embryo
Method/AssaySequencing
Crispr efficiency 50.50%
PRM/crRNA/VectorPCR8/GW/TOPO/TA
PRM-cas/vectorpHsp70
Pubmed24257628