A Central Hub of Crispr based Genome Editing
CrisprID | Crispr2180 |
Organism | Danio rerio |
Gene/Locus (Literature) | C13H9orf72(C9t3) |
NCBI Gene Name | C13H9orf72(C9t3) |
Target Sequence | CCTATCATGTCTTCAGCCTGTCC |
Target+Mutant_Flanking | TATAAGCCCCTATCATGTCTTCAGCCTGTCCTCCACAATCTCCAGCCGTGGCC |
Modification Length | D1 |
Genetic Modification | Deletion |
Location | Intron1 |
Conc. (ng/μl) | 2.4/0.5 |
Cell line | Embryo |
Method/Assay | Sequencing |
Crispr efficiency | 50.50% |
PRM/crRNA/Vector | PCR8/GW/TOPO/TA |
PRM-cas/vector | pHsp70 |
Pubmed | 24257628 |