CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr2197 details


CrisprIDCrispr2197
OrganismNicotiana benthamiana
Gene/Locus (Literature)PDS
NCBI Gene NamePDS
Target SequenceGCCGTTAATTTGAGAGTCCAAGG
Target+Mutant_Flanking

AATGCCCCAAATTGGACTTGTTTCTGCCGTTAATTTGAGAGT - CCAAGGTAATTCAGCTTATCTTTGGAGCTCGAGGTC
AATGCCCCAAATTGGACTTGTTTCTGCCGTTAATTT - - - - - T - CCAAGGTAATTCAGCTTATCTTTGGAGCTCGAGGTC

Modification LengthD5
Genetic ModificationDeletion
LocationNA
Conc. (ng/μl) NA
Cell line Leaf
Method/AssaySequencing/PCR
Crispr efficiency 2.10%
PRM/crRNA/VectorAtU6p
PRM-cas/vectorpENTR/D-TOPO
Pubmed23929340