CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr2203 details


CrisprIDCrispr2203
OrganismOryza sativa
Gene/Locus (Literature)PDS
NCBI Gene NamePDS
Target SequenceGTTGGTCTTTGCTCCTGCAGAGG
Target+Mutant_Flanking

TCCAAACCGTTCAATGCTGGAGTTGGTCTTTGCTCCTGCAGAGGAATGGGTTGGACGGAGTGAC
TCCAAACCGTTCAATGCTGGAGTTGGTCTTTG
- - - - - - CAGAGGAATGGGTTGGACGGAGTGAC

Modification LengthD6
Genetic ModificationDeletion
LocationNA
Conc. (ng/μl) NA
Cell line Protoplast
Method/AssayPCR/RE
Crispr efficiency 20%
PRM/crRNA/VectorOsU3/TaU6
PRM-cas/vectorpJIT163-2NLSCas9
Pubmed23929338