CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr2220 details


CrisprIDCrispr2220
OrganismOryza sativa
Gene/Locus (Literature)OsBADH2
NCBI Gene NameBADH2
Target SequenceGCAGATCTTGCAGAATCCTTGG
Target+Mutant_Flanking

CTGGATGCTTTGAGTACTTTGCAGATCTTGCAGAATCCTTGGACAAAAGGCAAAATGCACCT
CTGGATGCTTTGAGTACTTTGCA
- - -- - TGCAGAATCCTTGGACAAAAGGCAAAATGCACCT

Modification LengthD5
Genetic ModificationDeletion
LocationNA
Conc. (ng/μl) NA
Cell line Protoplast
Method/AssayPCR/RE
Crispr efficiency 26.50%
PRM/crRNA/VectorOsU3/TaU6
PRM-cas/vectorpJIT163-2NLSCas9
Pubmed23929338