CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr2229 details


CrisprIDCrispr2229
OrganismTriticum aestivum
Gene/Locus (Literature)TaMLO
NCBI Gene NameTaMLO
Target SequenceCCGTCACGCAGGACCCAATCTCC
Target+Mutant_Flanking

ATTCATCTCGCTGCTGCTCGCCGTCACGCAGGACCCAATCTCCGGGATATGCATCTCCCAGAA
ATTCATCTCGCTGCTGCTCG
CCGTCACGCAGGA - - - - ATCTCCGGGATATGCATCTCCCAGAA

Modification LengthD4
Genetic ModificationDeletion
LocationNA
Conc. (ng/μl) NA
Cell line Protoplast
Method/AssayPCR/RE
Crispr efficiency 28.50%
PRM/crRNA/VectorOsU3/TaU6
PRM-cas/vectorpJIT163-2NLSCas9
Pubmed23929338