CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr2325 details


CrisprIDCrispr2325
OrganismArabidopsis thaliana
Gene/Locus (Literature)BRl1
NCBI Gene NameBRl1
Target SequenceTTGGGTCATAACGATATCTCTGG
Target+Mutant_Flanking

AGATTGGTTCGATGCCTTATCTGTTTATTCTCAATTTGGGTCATAACGAT - - - - - A- - - - - - - - - T - - - - - C - - - TC - TGGTTCGATTCCTGATGAGGTAGGTGATCTA
AGATTGGTTCGATGCCTTATCTGTTTATTCTCAATTTGGGTCATAACGAT - - - - - A - - - - - - - - - - - - - - - - - - - - - - - TGGTTCGATTCCTGATGAGGTAGGTGATCTA

Modification LengthD4
Genetic ModificationDeletion
LocationNA
Conc. (ng/μl) NA
Cell line Protoplast
Method/AssayRFLP
Crispr efficiency 84%
PRM/crRNA/VectorAtU6-26
PRM-cas/vectorCaMV 35S
Pubmed23958582