A Central Hub of Crispr based Genome Editing
| CrisprID | Crispr2459 |
| Organism | Caenorhabditis elegans |
| Gene/Locus (Literature) | pie-1 |
| NCBI Gene Name | pie-1 |
| Target Sequence | GGCTCAGATTGACGAGGCGCCGG |
| Target+Mutant_Flanking | CCATTGGCTCAGATTGACGAGGCGCCGGCAACTAAAAGA |
| Modification Length | D5 |
| Genetic Modification | Deletion (Homozygotes) |
| Location | NA |
| Conc. (ng/μl) | 50/50 |
| Cell line | gonads of adult worm |
| Method/Assay | PCR,PAGE,Sequencing |
| Crispr efficiency | 55% |
| PRM/crRNA/Vector | U6 |
| PRM-cas/vector | Peft3::Cas9 |
| Pubmed | 24879462 |