CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr2477 details


CrisprIDCrispr2477
OrganismCaenorhabditis elegans
Gene/Locus (Literature)avr-15
NCBI Gene Nameavr-15
Target SequenceCCTGGGTGACTTATATTCGTTTG
Target+Mutant_Flanking

GAGTTCGCCTGGGTGACTTATATTCGTTTG
GAGTTCGCCTGGGTGA*CTTATATTCGTTTG (*tc)

Modification Length2
Genetic Modificationinsertion
LocationNA
Conc. (ng/μl) 50/50
Cell line gonads of adult worm
Method/AssayPCR,PAGE,Sequencing
Crispr efficiency 88%
PRM/crRNA/VectorU6
PRM-cas/vectorPeft3::Cas9
Pubmed24879462