CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr2521 details


CrisprIDCrispr2521
OrganismBombyx mori
Gene/Locus (Literature)BmKu70
NCBI Gene NameKu70
Target SequenceCCACCTCTGTCTGAGGATGAAAC
Target+Mutant_Flanking

ACACCACCTCTGTCTGAGGATGAAACACC
ACA
CCACCT - - - - - - - - - - - - -- - - - - - - -

Modification LengthD27
Genetic ModificationDeletion
LocationExon2
Conc. (ng/μl) 250
Cell line BmN
Method/AssayT7E1 assay
Crispr efficiency 30.30%
PRM/crRNA/VectorpUC57-T
PRM-cas/vectorpUC57-T
Pubmed24671069