CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr2535 details


CrisprIDCrispr2535
OrganismBombyx mori
Gene/Locus (Literature)BmKu70
NCBI Gene NameKu70
Target SequenceCCCACAAATTGCGCATGTTGCAACGTG - - - 105 - - - TATGCCATTGGCGCCACCTAACATGGATG - - - 152 - - - ACACCACCTCTGTCTGAGGATGAAAC
Target+Mutant_Flanking

TTCCCACAAATTGCGCATGTTGCAACGTG - - - 105 - - - TATGCCATTGGCGCCACCTAACATGGATG - - - 152 - - - ACACCACCTCTGTCTGAGGATGAAACACC
TTCCCACAAATTGCGCATGTTGCAACGTG -- - 105 - - - TATGCCATTGGCGCCACCT - ACATGGATG - - - 152 - - - ACACCACCTCTGTCTGAGGATGTAACACC

Modification LengthNA
Genetic ModificationDeletion
LocationExon2
Conc. (ng/μl) 250
Cell line BmN
Method/AssayT7E1 assay
Crispr efficiency NA
PRM/crRNA/VectorpUC57-T
PRM-cas/vectorpUC57-T
Pubmed24671069