CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr2545 details


CrisprIDCrispr2545
OrganismHomo sapiens
Gene/Locus (Literature)DNMT3B
NCBI Gene NameDNMT3B
Target SequenceTGCTGAATTACTCACGCCCCAAGG
Target+Mutant_Flanking

TTCGAATTTTACCACCTGCTGAATTACTCACGCCCCAAGGAGGGTGATGACCGGCCGTTC
TTCGAATTTTACCACCTGCTGAATTACTCACGCCCC - - - - - - - - - - ATGACCGGCCGTTC

Modification LengthD10
Genetic ModificationDeletion
LocationExon19
Conc. (ng/μl) NA
Cell line HiPSC
Method/AssaySequencing/PCR
Crispr efficiency 63%
PRM/crRNA/VectorU6
PRM-cas/vectorCAG
Pubmed24084724