CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr2571 details


CrisprIDCrispr2571
OrganismDanio rerio
Gene/Locus (Literature)etsrp
NCBI Gene Nameetv2
Target SequenceGAACTCAGGAGGTTCCTGCTGG
Target+Mutant_Flanking

GAACTCAGGAGGTTCCTGCTGGTTTCG
GAACTCAGGAGGTTCCA
ATTCTGCTGGTTTCGACTTCAGT- CG

Modification Length5
Genetic Modificationinsertion
LocationExon2
Conc. (ng/μl) 20/300
Cell line Embryo
Method/AssaySSA recombination assay
Crispr efficiency 31.2-38%
PRM/crRNA/VectorU6/PXT7
PRM-cas/vectorCMV/pUC19
Pubmed23528705