CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr2576 details


CrisprIDCrispr2576
OrganismDanio rerio
Gene/Locus (Literature)gata5
NCBI Gene Namegata5
Target SequenceGACTGTCGAATAAGAGAAACCGGGCGGTGG
Target+Mutant_Flanking

GACTGTCGAATAAGAGAAACCGGGCGGTGGATGAGGGC
GACTGTCGAATAT - - -- - - - - - G - - - - TGG 

Modification LengthD13
Genetic ModificationDeletion
LocationExon2
Conc. (ng/μl) 20/300
Cell line Embryo
Method/AssaySSA recombination assay
Crispr efficiency 50%-90%
PRM/crRNA/VectorU6/PXT7
PRM-cas/vectorCMV/pUC19
Pubmed23528705